Ciarloni, Laura Ehrensberger, Sahar Hosseinian Imaizumi, Natsuko Monnier-Benoit, Sylvain Nichita, Cristina Myung, Seung-Jae Kim, Joo Sung Song, Si Young Kim, Tae Il Weg, Boudewijn van der Meier, Rémy Borovicka, Jan Beglinger, Christoph Vallet, Cédric Maerten, Philippe Rüegg, Curzio Dorta, Gian 2016-04-28 A blood test for colorectal cancer (CRC) screening is a valuable tool to for testing asymptomatic individuals and reducing CRC-related mortality. The objective of this study was to develop and validate a novel blood test able to differentiate patients with CRC and adenomatous polyps (AP) from individuals with a negative colonoscopy. Experimental Design: A case-control, multicenter clinical study was designed to collect blood samples from patients referred for colonoscopy or surgery. Predictive algorithms were developed on 75 controls, 61 large AP (LAP) {greater than or equal to}1cm, 45 CRC, and independently validated on 74 controls, 42 LAP, 52 CRC (23 Stages I-II) as well as on 245 cases including other colorectal findings and diseases other than CRC. The test is based on a 29-gene panel expressed in peripheral blood mononuclear cells alone or in combination with established plasma tumor markers. Results: The 29-gene algorithm detected CRC and LAP with a sensitivity of 79.5% and 55.4%, respectively, with 90.0% specificity. Combination with the protein tumor markers CEA and CYFRA21-2 resulted in a specificity increase (92.2%) with a sensitivity for CRC and LAP detection of 78.1% and 52.3%, respectively. Conclusions: We report the validation of a novel blood test, Colox®, for the detection of CRC and LAP based on a 29-gene panel and the CEA and CYFRA21-1 plasma biomarkers. The performance and convenience of this routine blood test provides physicians a useful tool to test average risk individuals unwilling to undergo upfront colonoscopy. application/pdf https://folia.unifr.ch/global/documents/304891 https://folia.unifr.ch/documents/304891/files/rue_dcv.pdf https://folia.unifr.ch/documents/304891/files/rue_dcv_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1158/1078-0432.CCR-15-2057 info:eu-repo/semantics/openAccess License undefined Clinical Cancer Research. - 2016, vol. 22, no. 18, p. 4604-4611 info:eu-repo/classification/udc/57 Development and clinical validation of a blood test based on 29-gene expression for early detection of colorectal cancer http://purl.org/coar/resource_type/c_6501