Nordmann, Patrice Jayol, Aurélie Poirel, Laurent 2016 For identification of polymyxin resistance in Enterobacteriaceae, we developed a rapid test that detects glucose metabolization associated with bacterial growth in the presence of a defined concentration of colistin or polymyxin B. Formation of acid metabolites is evidenced by a color change (orange to yellow) of a pH indicator (red phenol). To evaluate the test, we used bacterial colonies of 135 isolates expressing various mechanisms of colistin resistance (intrinsic, chromosomally encoded, and plasmid-mediated MCR-1) and 65 colistin-susceptible isolates. Sensitivity and specificity were 99.3% and 95.4%, respectively, compared with the standard broth microdilution method. This new test is inexpensive, easy to perform, sensitive, specific, and can be completed in <2 hours. It could be useful in countries facing endemic spread of carbapenemase producers and for which polymyxins are last-resort drugs. application/pdf https://folia.unifr.ch/global/documents/304829 https://folia.unifr.ch/documents/304829/files/nor_rdp.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3201/eid2206.151840 info:eu-repo/semantics/openAccess License undefined Emerging Infectious Diseases. - 2016, vol. 22, no. 6, p. 1038-1043 info:eu-repo/classification/udc/57 Rapid detection of polymyxin resistance in Enterobacteriaceae http://purl.org/coar/resource_type/c_6501