Santoro, Giuseppe Beltrami, Ruben Kottela, Emmanuel Blacque, Olivier Bogdanova, Anna Yu. Zobi, Fabio 2016-01-19 Mixed CO/NO-releasing molecules were prepared by conjugation of the 17-electron rhenium dicarbonyl <em>cis</em>-[Re(CO)₂Br₄]²⁻ complex to <em>N</em>-nitrosamine modified cyanocobalamin (B12) bio-vectors. The species were fully characterized by standard analytical techniques, including X-ray crystallography for cyanocobalamin-5′-<em>O</em>-pyrazine and (<strong>1</strong>) and its <em>N</em>-pyrazine nitrosylated derivative (<strong>1a</strong>). The <em>N</em>-nitrosamine B12 derivatives are able to liberate low NO doses in buffer solutions and appear to be “activated” towards NO release if in contact with cultured cells. Coordination of the <em>cis</em>-[Re(CO)₂Br₄]²⁻ complex on the axial cyanide of B12 allows for the combined loss of CO and NO from the conjugates. The mixed CO/NO-releasing molecules show cytoprotection in an ischemia–reperfusion model but no significant enhanced synergistic effects over the relative NORMs and CORMs building constituents. application/pdf https://folia.unifr.ch/global/documents/304720 https://folia.unifr.ch/documents/304720/files/zob_ncc.pdf https://folia.unifr.ch/documents/304720/files/zob_ncc_sm.pdf https://folia.unifr.ch/documents/304720/files/zob_ncc_sm2.cif eng info:eu-repo/semantics/altIdentifier/doi/10.1039/C5DT03402G info:eu-repo/semantics/openAccess License undefined Dalton Transactions. - 2016, vol. 45, no. 4, p. 1504–1513 info:eu-repo/classification/udc/54 N-Nitrosamine-{cis--Re[CO]₂}²⁺ cobalamin conjugates as mixed CO/NO-releasing molecules http://purl.org/coar/resource_type/c_6501