Kuhn, Dagmar A. Hartmann, Raimo Fytianos, Kleanthis Petri-Fink, Alke Rothen-Rutishauser, Barbara Parak, Wolfgang J. 2015-06-01 Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from dendritic cells by a monolayer of epithelial cells. While passing of small nanoparticles has been demonstrated from macrophages to dendritic cells across the epithelial barrier in previous studies, for the micrometer-sized capsules, this process could not be observed in a significant amount. Thus, this barrier is a limiting factor for cell-to-cell transfer of micrometer-sized particles. application/pdf https://folia.unifr.ch/global/documents/304491 https://folia.unifr.ch/documents/304491/files/fin_cuc.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1088/1468-6996/16/3/034608 info:eu-repo/semantics/openAccess License undefined Science and Technology of Advanced Materials. - 2015, vol. 16, no. 3, p. 034608 info:eu-repo/classification/udc/54 Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract http://purl.org/coar/resource_type/c_6501