Santoro, Giuseppe Zlateva, Theodora Ruggi, Albert Quaroni, Luca Zobi, Fabio 2015-04-01 Constitutional isomers of cyanocobalamin adducts based on a fluorescent rhenium tris-carbonyl diimine complex were prepared, characterized and tested against PC-3 cancer cells. The adducts differ only in the relative binding position of the organometallic species which is either bound at the cyano or the 5′-hydroxo group of vitamin B₁₂. When tested for their cytotoxic potency, the species showed IC₅₀ values in the low μM rage. Upon conjugation to the vitamin an energy transfer process causes an extremely low quantum yield of fluorescence emission, making the conjugates unsuitable for fluorescence imaging. However, by exploiting the vibrational signature of the <em>fac</em>-[Re(CO)₃]⁺ core, their cellular distribution was evaluated <em>via</em> FTIR spectromicroscopy. application/pdf https://folia.unifr.ch/global/documents/304417 https://folia.unifr.ch/documents/304417/files/san_scc.pdf https://folia.unifr.ch/documents/304417/files/san_scc_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1039/C4DT03598D info:eu-repo/semantics/openAccess License undefined Dalton Transactions. - 2015, vol. 44, no. 15, p. 6999–7008 info:eu-repo/classification/udc/54 Synthesis, characterization and cellular location of cytotoxic constitutional organometallic isomers of rhenium delivered on a cyanocobalmin scaffold http://purl.org/coar/resource_type/c_6501