Fytianos, Kleanthis Rodriguez-Lorenzo, Laura Clift, Martin J. D. Blank, Fabian Vanhecke, Dimitri Garnier, Christophe von Petri-Fink, Alke Rothen-Rutishauser, Barbara 2015 Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties. application/pdf https://folia.unifr.ch/global/documents/304256 https://folia.unifr.ch/documents/304256/files/fin_ues.pdf https://folia.unifr.ch/documents/304256/files/fin_ues_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nano.2014.11.004 info:eu-repo/semantics/openAccess License undefined Nanomedicine: Nanotechnology, Biology and Medicine. - 2015, vol. 11, no. 3, p. 633–644 info:eu-repo/classification/udc/54 Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro http://purl.org/coar/resource_type/c_6501