Charfi-Kessis, Karama Mansour, Wejdene Khalifa, Anis Ben Haj Mastouri, Maha Nordmann, Patrice Aouni, Mahjoub Poirel, Laurent 2014-07-22 Multidrug-resistant and difficult-to-treat <em>Acinetobacter baumannii</em> may be responsible for nosocomial infections. The production of carbapenem-hydrolyzing class D β-lactamases (CHDLs) and extended-spectrum β-lactamase (ESBLs) of the GES type possessing a carbapenemase activity has been increasingly reported worldwide in <em>A. baumannii</em>. The aim of this study was to analyze the resistance mechanisms of two carbapenem resistant <em>A. baumannii</em> clinical isolates recovered in a neonatology center in the center-east of Tunisia.Two carbapenem resistant <em>A. baumannii</em> isolates were recovered. The first isolate co-harbored the <em>bla</em>_GES-11 ESBL gene and the <em>bla</em>_O_xA-23 CHDL gene. Analyses of the genetic location indicated that the <em>bla</em>_GES-11 gene was plasmid located (Gr6). However, the <em>bla</em>_O_xA-23 gene was located on the chromosome. The second strain had only the <em>bla</em>_O_xA-23 CHDL gene, which was plasmid located.This study showed the first description of the GES-type β-lactamase in <em>A. baumannii</em> in Tunisia. application/pdf https://folia.unifr.ch/global/documents/303675 https://folia.unifr.ch/documents/303675/files/nor_mra.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2014.07.003 info:eu-repo/semantics/openAccess License undefined Microbial Pathogenesis. - 2014, vol. 74, p. 20–24 Acinetobacter baumannii GES 11 OXA 23 Carbapenem resistance Neonatology center Tunisia info:eu-repo/classification/udc/57 Multidrug-resistant Acinetobacter baumannii strains carrying the blaOxA-23 and the blaGES-11 genes in a neonatology center in Tunisia http://purl.org/coar/resource_type/c_6501