Silva, Yussuan Portieles, Roxana Pujol, Merardo Terauchi, Ryohei Matsumura, Hideo Serrano, Mario Borrás-Hidalgo, Orlando 2013-08-15 In order to identify Nicotiana megalosiphon genes for novel inhibitors of microbial serine proteinase involved in resistance to the oomycete Phytophthora parasitica var. nicotianae, SuperSAGE technology combined with next-generation sequencing were used to generate libraries in order to identify transcripts that are differentially up-regulated. We identified a N. megalosiphon inhibitor of microbial serine proteinase gene (NmIMSP) rapidly induced during the interaction. Silencing of NmIMSP gene was found to compromise the resistance of N. megalosiphon to P. parasitica var. nicotianae. Herewith, the transient expression of NmIMSP gene in a susceptible genotype conferred high level of resistance to P. parasitica var. nicotianae and Peronospora hyoscyami f. sp. tabacina. This is the first evidence of this inhibitor of microbial serine proteinase as a positive regulator of defense response against oomycete. application/pdf https://folia.unifr.ch/global/documents/303524 https://folia.unifr.ch/documents/303524/files/serr_ems.pdf https://folia.unifr.ch/documents/303524/files/serr_ems_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pmpp.2013.08.001 info:eu-repo/semantics/openAccess License undefined Physiological and Molecular Plant Pathology. - 2013, vol. 84, p. 99–106 Proteinase inhibitor Nicotiana megalosiphon SuperSAGE Oomycete info:eu-repo/classification/udc/57 Expression of a microbial serine proteinase inhibitor gene enhances the tobacco defense against oomycete pathogens http://purl.org/coar/resource_type/c_6501