Zimmermann, Mirjam Cal, Ruben Janett, Elia Hoffmann, Viktor Bochet, Christian G. Constable, Edwin Beaufils, Florent Wymann, Matthias P. 2014-04-25 Chemical inducers of dimerization (CIDs) have been developed to orchestrate protein dimerization and translocation. Here we present a novel photocleavable HaloTag- and SNAP-tag-reactive CID (MeNV-HaXS) with excellent selectivity and intracellular reactivity. Excitation at 360 nm cleaves the methyl-6-nitroveratryl core of MeNV-HaXS. MeNV-HaXS covalently links HaloTag- and SNAP-tag fusion proteins, and enables targeting of selected membranes and intracellular organelles. MeNV-HaXS-mediated translocation has been validated for plasma membrane, late endosomes, lysosomes, Golgi, mitochondria, and the actin cytoskeleton. Photocleavage of MeNV-HaXS liberates target proteins and provides access to optical manipulation of protein relocation with high spatiotemporal and subcellular precision. MeNV-HaXS supports kinetic studies of protein dynamics and the manipulation of subcellular enzyme activities, which is exemplified for Golgi-targeted cargo and the assessment of nuclear import kinetics. application/pdf https://folia.unifr.ch/global/documents/303459 https://folia.unifr.ch/documents/303459/files/boc_cpp.pdf https://folia.unifr.ch/documents/303459/files/boc_cpp_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1002/anie.201310969 info:eu-repo/semantics/openAccess License undefined Angewandte Chemie International Edition. - 2014, vol. 53, no. 18, p. 4717–4720 chemical inducers of dimerization fusion proteins photocleavable linkers photolysis protein protein interactions info:eu-repo/classification/udc/54 Cell-Permeant and photocleavable chemical inducer of dimerization http://purl.org/coar/resource_type/c_6501