Girlich, D. Bouihat, N. Poirel, Laurent Benouda, A. Nordmann, Patrice 2013-08-09 Carbapenemase-producing Enterobacteriaceae isolates are being increasingly reported, particularly from countries surrounding the Mediterranean area. We aimed to quantify the prevalence of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in rectal swabs from hospitalized patients in a University hospital in Morocco, and to compare the performance of three screening media: ChromID ESBL (bioMérieux), Brilliance CRE (OXOID, Thermofisher) and SUPERCARBA (home made). Genetic detection and plasmid analysis were performed by PCR and sequencing. Strain comparison was performed by multi-locus sequence typing and the Diversilab technique (bioMérieux). The prevalence of multidrug-resistant Enterobacteriaceae was high, with 33 ESBL producers (42.85%, mainly CTX-M-15) and 10 OXA-48 producers (13%), corresponding to two major clones of K. pneumoniae (70%) and a clone of Enterobacter cloacae (30%). The three screening media showed the same sensitivity for detection of carbapenemase-producing Enterobacteriaceae, whereas the SUPERCARBA medium was more specific than the two other media. The average faecal carriage of ESBL or carbapenemase-producing Enterobacteriaceae varied from 1 × 102 to >1 × 108 CFU/g of stools. This study shows a high prevalence of multidrug-resistant Enterobacteriaceae, and particularly of OXA-48 producers. The new carbapenem-containing medium, SUPERCARBA, was as sensitive as Brilliance CRE and ChromID ESBL, and more specific for the detection of Enterobacteriaceae expressing those carbapenemases. application/pdf https://folia.unifr.ch/global/documents/303109 https://folia.unifr.ch/documents/303109/files/nor_hrf.pdf https://folia.unifr.ch/documents/303109/files/nor_hrf_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1111/1469-0691.12325 info:eu-repo/semantics/openAccess License undefined Clinical Microbiology and Infection. - 2014, vol. 20, no. 4, p. 350-354 Multidrug resistant bacteria prevalence stools info:eu-repo/classification/udc/616 High rate of faecal carriage of extended-spectrum β-lactamase and OXA-48 carbapenemase-producing Enterobacteriaceae at a University hospital in Morocco http://purl.org/coar/resource_type/c_6501