Bhattacharyya, Anwesha Bießmann, Felix Veit, Julia Kretz, Robert Rainer, Gregor 2012-04-04 Acetylcholine is an important neuromodulator involved in cognitive function. The impact of cholinergic neuromodulation on computations within the cortical microcircuit is not well understood. Here we investigate the effects of layer-specific cholinergic drug application in the tree shrew primary visual cortex during visual stimulation with drifting grating stimuli of varying contrast and orientation. We describe differences between muscarinic and nicotinic cholinergic effects in terms of both the layer of cortex and the attribute of visual representation. Nicotinic receptor activation enhanced the contrast response in the granular input layer of the cortex, while tending to reduce neural selectivity for orientation across all cortical layers. Muscarinic activation modestly enhanced the contrast response across cortical layers, and tended to improve orientation tuning. This resulted in highest orientation selectivity in the supra- and infragranular layers, where orientation selectivity was already greatest in the absence of pharmacological stimulation. Our results indicate that laminar position plays a crucial part in functional consequences of cholinergic stimulation, consistent with the differential distribution of cholinergic receptors. Nicotinic receptors function to enhance sensory representations arriving in the cortex, whereas muscarinic receptors act to boost the cortical computation of orientation tuning. Our findings suggest close homology between cholinergic mechanisms in tree shrew and primate visual cortices. application/pdf https://folia.unifr.ch/global/documents/302423 https://folia.unifr.ch/documents/302423/files/kre_fld.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2012.08052.x info:eu-repo/semantics/openAccess License undefined European Journal of Neuroscience. - 2012, vol. 35, no. 8, p. 1270-1280 Acetylcholine contrastgain neuralcomputation Nicotine orientationtuning Oxotremorine info:eu-repo/classification/udc/57 Functional and laminar dissociations between muscarinic and nicotinic cholinergic neuromodulation in the tree shrew primary visual cortex http://purl.org/coar/resource_type/c_6501