Bessire, Michael Borel, Sandra Fabre, Guillaume Carraça, Luis Efremova, Nadia Yephremov, Alexander Cao, Yan Jetter, Reinhard Jacquat, Anne-Claude Métraux, Jean-Pierre Nawrath, Christiane 2011-05-31 Although the multilayered structure of the plant cuticle was discovered many years ago, the molecular basis of its formation and the functional relevance of the layers are not understood. Here, we present the <em>permeable cuticle1</em> (<em>pec1</em>) mutant of <em>Arabidopsis thaliana</em>, which displays features associated with a highly permeable cuticle in several organs. In <em>pec1</em> flowers, typical cutin monomers, such as ω- hydroxylated fatty acids and 10,16-dihydroxypalmitate, are reduced to 40% of wild-type levels and are accompanied by the appearance of lipidic inclusions within the epidermal cell. The cuticular layer of the cell wall, rather than the cuticle proper, is structurally altered in <em>pec1</em> petals. Therefore, a significant role for the formation of the diffusion barrier in petals can be attributed to this layer. Thus, <em>pec1</em> defines a new class of mutants. The phenotypes of the <em>pec1</em> mutant are caused by the knockout of ATP BINDING CASSETTEG32 (ABCG32), an ABC transporter from the PLEIOTROPIC DRUG RESISTANCE family that is localized at the plasma membrane of epidermal cells in a polar manner toward the surface of the organs. Our results suggest that ABCG32 is involved in the formation of the cuticular layer of the cell wall, most likely by exporting particular cutin precursors from the epidermal cell. application/pdf https://folia.unifr.ch/global/documents/302117 https://folia.unifr.ch/documents/302117/files/met_mpd.pdf https://folia.unifr.ch/documents/302117/files/met_mpd_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.111.083121 info:eu-repo/semantics/openAccess License undefined Plant Cell. - 2011, vol. 23, no. 5, p. 1958-1970 info:eu-repo/classification/udc/57 A member of the PLEIOTROPIC DRUG RESISTANCE family of ATP binding cassette transporters is required for the formation of a functional cuticle in Arabidopsis http://purl.org/coar/resource_type/c_6501