Hernadfalvi, Noemi Langhans, Wolfgang Meyenburg, Claudia von Onteniente, Brigitte Richard, Denis Arsenijevic, Denis 2007 To study the role of the redox state regulator glutathione (GSH) in bacterial lipopolysaccharide (LPS)-induced anorexia we measured GSH in liver, serum and brain in response to intraperitoneal (ip) lipopolysaccharide (LPS, 4μg/mouse) injection in LPS-naïve and LPS-pretreated (4 μg/mouse) mice. LPS reduced food intake in LPS-naïve mice and LPS pretreatment attenuated this effect. LPS reduced total reduced GSH at 24 hours after injection in LPS-naïve mice. On the other hand, LPS pretreatment caused an upregulation of GSH levels in brain and liver, and this was associated with a significant attenuation of LPS-induced anorexia. In addition, LPS pretreatment increased mitochondrial GSH levels in brain and liver. Pharmacological GSH depletion with diethylmaleate and L-buthionine sulfoximine in LPS-pretreated mice ablated the hyposensitivity to the anorexic effect of LPS. Together, these findings suggest a prominent role for GSH and its intracellular repartition in LPS anorexia. application/pdf https://folia.unifr.ch/global/documents/300578 https://folia.unifr.ch/documents/300578/files/arsenijevic_rgh.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1684/ecn.2007.0090 info:eu-repo/semantics/openAccess License undefined European Cytokine Network. - 2007, vol. 18, no. 2, p. 39-45 reactive oxygen species cytokine food intake liver brain mitochondria info:eu-repo/classification/udc/57 Role for glutathione in the hyposensitivity of LPS-pretreated mice to LPS anorexia http://purl.org/coar/resource_type/c_6501