Arsenijevic, Denis Clavel, Sébastien Sanchis, Daniel Plamondon, Julie Huang, Quingling Ricquier, Daniel Rouger, Laurie Richard, Denis 2007-04-30 A model of murine toxoplasmosis was used to study cellular and temporal expression of uncoupling protein-2 (<i>Ucp2</i>) in the brain. <i>In situ</i> hybridization indicated that <i>Ucp2</i> was located in neurons. Nuclei structures involved in energy balance, in particular the nucleus of the solitary tract (NST), was shown to have a positive association between negative energy balance and <i>Ucp2</i> levels. Infection-induced <i>Ucp2</i> expression colocalized mainly with microglial cells, but also with infiltrating macrophages and neutrophils in the brain, which was evident from day 9 post-infection. Using cytokine knockout mice we demonstrate that microglial <i>Ucp2</i> induction in the brain was largely dependant on interferon-γ, but not interleukin-6 or tumour-necrosis-factor-α in response to infection. In summary, this study shows that <i>Ucp2</i> is regulated in a different manner in neurons than in microglia/phagocytes following infection. Our study indicates that an association exists between negative energy balance and neuronal <i>Ucp2</i> levels in the NST, in particular. application/pdf https://folia.unifr.ch/global/documents/300577 https://folia.unifr.ch/documents/300577/files/arsenijevic_iue.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneuroim.2007.03.013 info:eu-repo/semantics/openAccess License undefined Journal of Neuroimmunology. - 2007, vol. 186, no. 1-2, p. 121-132 uncoupling-protein-2 toxoplasmosis brain neuron microglia interferon-gamma info:eu-repo/classification/udc/57 Induction of Ucp2 expression in brain phagocytes and neurons following murine toxoplasmosis: An essential role of IFN-γ and an association with negative energy balance http://purl.org/coar/resource_type/c_6501