Phuntumart, Vipaporn Marro, Pascal Métraux, Jean-Pierre Sticher, Liliane 2006-07-05 Several genes were isolated by differential display of mRNAs from cucumber leaves inoculated with the bacterium, <i>Pseudomonas syringae</i> pv. <i>lachrymans</i>. A full-length cDNA encoding a novel pathogen-induced gene, <i>Cupi4</i>, was cloned and characterized in detail. While <i>Cupi4</i> did not share evident homology with known sequences in the database at the nucleotide level, the predicted amino acid sequence of <i>Cupi4</i> shared homology with the pathogen-inducible proteins, pMB57-10G 5′ of <i>Brassica napus</i> (21%) and <i>CXc750</i>/<i>ESC1</i> of <i>Arabidopsis thaliana</i> (16%). <i>Cupi4</i> transcripts accumulated after 12 h in leaves inoculated with <i>P. s. lachryman</i>s and after 48 h in the systemic upper leaves of the inoculated plants. Treatment with the chemical inducers of systemic acquired resistance (SAR), salicylic acid, 2,6-dichloroisonicotinic acid and benzothiadiazole as well as inoculation with different pathogens, <i>P. s. syringae</i>, <i>Colletotrichum lagenarium</i> and tobacco necrosis virus also led to the accumulation of <i>Cupi4</i> transcripts. The increase of <i>Cupi4</i> transcripts in both the inoculated first leaf and in systemic upper leaves suggested that the <i>Cupi4</i> gene product is associated with systemic acquired resistance in cucumber. Induced expression of CUPI4 in different host strains of a bacterium, <i>Escherichia coli</i>, led to death of bacterial host cells, suggesting that CUPI4 might have antibacterial properties. application/pdf https://folia.unifr.ch/global/documents/300219 https://folia.unifr.ch/documents/300219/files/sticher_ncg.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2006.05.014 info:eu-repo/semantics/openAccess License undefined Plant Science. - 2006, vol. 171, no. 5, p. 555–564 class III chitinase Cucumber Cupi4 Pseudomonas syringae pv. lachrymans pathogen-induced proteins systemic acquired resistance info:eu-repo/classification/udc/57 A novel cucumber gene associated with systemic acquired resistance http://purl.org/coar/resource_type/c_6501