<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Parisy, Vincent</dc:creator>
  <dc:creator>Poinssot, Benoit</dc:creator>
  <dc:creator>Owsianowski, Lucas</dc:creator>
  <dc:creator>Buchala, Antony J.</dc:creator>
  <dc:creator>Glazebrook, Jane</dc:creator>
  <dc:creator>Mauch, Felix</dc:creator>
  <dc:date>2006</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">The Arabidopsis &lt;i&gt;pad2-1&lt;/i&gt; mutant belongs to a series of non-allelic camalexin-deficient mutants. It was originally described as showing enhanced susceptibility to virulent strains of &lt;i&gt;Pseudomonas syringae&lt;/i&gt; and was later shown to be hyper-susceptible to the oomycete pathogen &lt;i&gt;Phytophthora brassicae&lt;/i&gt; (formerly &lt;i&gt;P. porri&lt;/i&gt;). Surprisingly, in both pathosystems, the disease susceptibility of &lt;i&gt;pad2-1&lt;/i&gt; was not caused by the camalexin deficiency, suggesting additional roles of PAD2 in disease resistance. The susceptibility of &lt;i&gt;pad2-1&lt;/i&gt; to &lt;i&gt;P. brassicae&lt;/i&gt; was used to map the mutation to the gene At4g23100, which encodes &lt;i&gt;γ&lt;/i&gt;-glutamylcysteine synthetase (&lt;i&gt;γ&lt;/i&gt;-ECS, GSH1). GSH1 catalyzes the first committed step of glutathione (GSH) biosynthesis. The &lt;i&gt;pad2-1&lt;/i&gt; mutation caused an S to N transition at amino acid position 298 close to the active center. The conclusion that &lt;i&gt;PAD2&lt;/i&gt; encodes GSH1 is supported by several lines of evidence: (i) &lt;i&gt;pad2-1&lt;/i&gt; mutants contained only about 22% of wild-type amounts of GSH, (ii) genetic complementation of &lt;i&gt;pad2-1&lt;/i&gt; with wild-type &lt;i&gt;GSH1&lt;/i&gt; cDNA restored GSH production, accumulation of camalexin in response to &lt;i&gt;P. syringae&lt;/i&gt; and resistance to &lt;i&gt;P. brassicae&lt;/i&gt; and &lt;i&gt;P. syringae&lt;/i&gt;, (iii) another GSH1 mutant, &lt;i&gt;cad2-1&lt;/i&gt;, showed &lt;i&gt;pad2&lt;/i&gt;-like phenotypes, and (iv) feeding of GSH to excised leaves of &lt;i&gt;pad2-1&lt;/i&gt; restored camalexin production and resistance to &lt;i&gt;P. brassicae&lt;/i&gt;. Inoculation of Col-0 with &lt;i&gt;P. brassicae&lt;/i&gt; caused a coordinated increase in the transcript abundance of &lt;i&gt;GSH1&lt;/i&gt; and &lt;i&gt;GSH2&lt;/i&gt;, the gene encoding the second enzyme in GSH biosynthesis, and resulted in enhanced foliar GSH accumulation. The &lt;i&gt;pad2-1&lt;/i&gt; mutant showed enhanced susceptibility to additional pathogens, suggesting an important general role of GSH in disease resistance of Arabidopsis.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://folia.unifr.ch/global/documents/300191</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/300191/files/mauch_ipg.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2006.02938.x</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>License undefined</dc:rights>
  <dc:source>The Plant Journal. - 2007, vol. 49, no. 1, p. 159-172</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">camalexin</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Phytophthora brassicae</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Pseudomonas syringae</dc:subject>
  <dc:subject>info:eu-repo/classification/udc/57</dc:subject>
  <dc:title xmlns:ns4="xml" ns4:lang="en">Identification of PAD2 as a γ-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>