<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Bahi, Amine</dc:creator>
  <dc:creator>Boyer, Frederic</dc:creator>
  <dc:creator>Bussard, Gaelle</dc:creator>
  <dc:creator>Dreyer, Jean-Luc</dc:creator>
  <dc:date>2005-07-15</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">The dopamine D₃ receptor (D₃R) is an important pharmacotherapeutic target for its potential role in psychiatric disorders and drug dependence. To further explore its function in rats, a regulatable lentivirus, Lenti-D3, holding the rat D₃R cDNA, has been constructed as well as three nonregulatable lentiviruses, Lenti-D3-siRNA1, Lenti-D3-siRNA2 and Lenti-D3-siRNA3, expressing small hairpin RNAs, aimed at silencing D₃R expression and specifically targeted against different regions of the D₃R mRNA. &lt;i&gt;In vitro&lt;/i&gt;, Lenti-D3 expressed D₃R and could efficiently be blocked with Lenti-D3-Sils. These viruses were stereotaxically injected into the shell part of the nucleus accumbens (NAcc) and effects of passive cocaine delivery on locomotor activity were assessed. Manipulations of D₃R levels induced changes in the locomotor stimulant effects of cocaine as compared to control treatment. Suppression of dopamine (DA) D₃R in the NAcc by means of local knockdown (with Lenti-D3-Sils) increased locomotor stimulant effects, whereas its overexpression with Lenti-D3 drastically reduced them. The latter effects could be reversed when animals were fed doxycycline, which prevented lentiviral-mediated DA D₃R overexpression in the NAcc. Gene expression assessed by quantitative RT-PCR confirmed very efficient gene knockdown &lt;i&gt;in vivo&lt;/i&gt; in animals treated with Lenti-D3-Sils (&gt; 93% silencing of D₃R gene). Thus D₃R expression significantly contributes to behavioural changes associated with chronic cocaine delivery.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://folia.unifr.ch/global/documents/299871</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/299871/files/1_dreyer_sdd.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2005.04157.x</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>License undefined</dc:rights>
  <dc:source>European Journal of Neuroscience. - 2005, vol. 21, no. 12, p. 3415-3426</dc:source>
  <dc:subject>info:eu-repo/classification/udc/57</dc:subject>
  <dc:title xmlns:ns1="xml" ns1:lang="en">Silencing dopamine D3-receptors in the nucleus accumbens shell in vivo induces changes in cocaine-induced hyperlocomotion</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>