Bahi, Amine Boyer, Frederic Kolira, Manoj Dreyer, Jean-Luc 2005-02-04 The tetraspanin CD81 is induced in the mesolimbic dopaminergic pathway after cocaine administration. To further investigate its role, a regulatable lentivirus (Lenti-CD81) bearing the CD81 gene under the control of a tetracycline-inducible promoter and lentiviruses expressing short hairpin RNA (shRNA) targeted against CD81 (Lenti-CD81-shRNAs) have been prepared. Infection of HEK293T cells <i>in vitro</i> with Lenti-CD81-shRNAs resulted in 96.5% gene silencing (from quantitative real-time PCR(qRT-PCR) and immunocytochemistry). <i>In vivo</i> delivery of Lenti-CD81-shRNA into the nucleus accumbens or ventral tegmental area resulted in 91.3 and 94% silencing of endogenous CD81, respectively. Stereotaxic injection of Lenti-CD81 into these regions, resulting in CD81 overexpression, induced a four- to fivefold increase in locomotor activity after chronic cocaine administration, which returned to basal levels when Lenti-CD81-shRNA had been coinjected or when CD81 expression was blocked by doxycycline. Furthermore, silencing endogenous CD81 <i>in vivo</i> resulted in a significant decrease in locomotor activity over controls, again suppressing cocaine-induced behaviour. application/pdf https://folia.unifr.ch/global/documents/299853 https://folia.unifr.ch/documents/299853/files/1_dreyer_ivg.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2004.02961.x info:eu-repo/semantics/openAccess License undefined Journal of Neurochemistry. - 2005, vol. 92, no. 5, p. 1243-1255 info:eu-repo/classification/udc/57 In vivo gene silencing of CD81 by lentiviral expression of small interference RNAs suppresses cocaine-induced behaviour http://purl.org/coar/resource_type/c_6501