Solinas, Giovanni Summermatter, Serge Mainieri, Davide Gubler, Marcel Pirola, Luciano Wymann, Matthias P. Rusconi, Sandro Montani, Jean-Pierre Seydoux, Josiane 2004-11-11 We report here studies that integrate data of respiration rate from mouse skeletal muscle in response to leptin and pharmacological interference with intermediary metabolism, together with assays for phosphatidylinositol 3-kinase (PI3K) and AMP- activated protein kinase (AMPK). Our results suggest that the direct effect of leptin in stimulating thermogenesis in skeletal muscle is mediated by substrate cycling between de novo lipogenesis and lipid oxidation, and that this cycle requires both PI3K and AMPK signaling. This substrate cycling linking glucose and lipid metabolism to thermogenesis provides a novel thermogenic mechanism by which leptin protects skeletal muscle from excessive fat storage and lipotoxicity. application/pdf https://folia.unifr.ch/global/documents/299659 https://folia.unifr.ch/documents/299659/files/1_dulloo_del.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2004.10.066 info:eu-repo/semantics/openAccess License undefined FEBS Letters. - 2004, vol. 577, no. 3, p. 539-544 Obesity Diabetes Lipotoxicity Gluco-lipotoxicity Insulin resistance Phosphatidylinositol 3-kinase AMP-activated protein kinase Sterol regulatory element binding protein-1c info:eu-repo/classification/udc/61 The direct effect of leptin on skeletal muscle thermogenesis is mediated by substrate cycling between de novo lipogenesis and lipid oxidation http://purl.org/coar/resource_type/c_6501